Maus, Irena; Klocke, Michael; Derenkó, Jaqueline; Stolze, Yvonne; Beckstette, Michael; Jost, Carsten; Wibberg, Daniel; Blom, Jochen; Henke, Christian; Willenbücher, Katharina; Rumming, Madis; Rademacher, Antje; Pühler, Alfred ; Sczyrba, Alexander ; Schlüter, Andreas: Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed [...]. In: Environmental Microbiome. Jg.15 H. 1. 2020
Inhalt
- Results
- Background
- Results
- Biogas reactor performance and long-term microbial dynamics
- Cellulolytic / hydrolytic biofilm community structure
- MAG abundance in the cellulolytic/hydrolytic biofilms depending on the temperature and the OLR
- Functional potential of MAGs positively responding to increased OLR
- Transcriptional activity of MAGs in response to temperature and OLR
- Transcriptional activity of MAGs normalized to MAG abundances
- Transcriptional profiling of most active cellulolytic / hydrolytic biofilm MAGs
- Discussion
- Conclusions
- Methods
- Reactor set-up and sampling
- Extraction of total microbial genomic DNA
- Terminal restriction fragment length polymorphism (TRFLP) fingerprinting
- Next-generation-sequencing (NGS) of 16S rRNA gene amplicon libraries
- Microbial metagenome library preparation, NGS, and assembly of genomes from metagenome datasets
- Microbial metatranscriptome library preparation and NGS
- Metagenome and metatranscriptome sequence analysis
- Phylogenetic and functional analysis of the metagenome-assembled genomes
- Supplementary information
- Abbreviations
- Acknowledgements
- Authors’ contributions
- Funding
- Availability of data and materials
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Author details
- References
- Publisher’s Note