Holtgräwe, Daniela ; Rosleff Soerensen, Thomas; Hausmann, Ludger; Pucker, Boas ; Viehöver, Prisca; Töpfer, Reinhard; Weisshaar, Bernd: A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development [...]. In: Frontiers in Plant Science. Jg.11. 2020
Inhalt
- BAC Library
- BAC End Sequencing by Sanger Sequencing
- Processing of BAC End Sequences (BES)
- NGS Sequencing Library Preparation
- NGS Read Pre-Processing
- Assembly, Post-Processing, and Validation
- Calculation of Heterozygosity
- Genome-Wide Variant Detection
- Simple Sequence Repeat Detection
- SSR Marker Validation
- Locus-Guided Variant Calling and Marker Generation
- Amplimer Design for the Rpv14 Locus
- Plant Material, Bulk Set Up, DNA Extraction, and PCR
- Determination of SNV/Allele Ratios
- Results
- A Draft Assembly of the ‘Börner’ Genome Sequence
- BoeWGS1.0 Contigs Representing Different Haplophases
- Homozygous and Heterozygous Variant Frequencies
- SSR Detection for Marker Development
- Targeted Mapping of the Downy Mildew Resistance Rpv14 by Bulk Segregant Analysis
- Discussion
- Genome Sequencing
- Deduction of SSR Sequences for Marker Development
- Heterozygosity of ‘Börner’
- Application of Phased Contig Data for Targeted Mapping of the Downy Mildew Resistance Rpv14 of Vitis
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References