Vordemvenne, Thomas; Wähnert, Dirk; Koettnitz, Julian; Merten, Madlen; Fokin, Nadine; Becker, Andreas; Büker, Björn; Vogel, Asaria; Kronenberg, Daniel; Stange, Richard; Wittenberg, Gunther; Greiner, Johannes ; Hütten, Andreas; Kaltschmidt, Christian; Kaltschmidt, Barbara: Bone Regeneration: A Novel Osteoinductive Function of Spongostan by the Interplay between Its Nano- and Microtopography.. In: Cells. Jg.9 H. 3. 2020
Inhalt
- Introduction
- Materials and Methods
- Study Design
- Spongostan
- Collagen Fibrils
- SiO2 Nanoparticles and Coating of Spongostan
- Light Microscopy
- Scanning Electron Microscopy
- Osteogenic Differentiation of Human Stem Cells in Spongostan and Detection of Calcium Deposition
- Immunocytochemistry
- Animals
- Animal Surgery
- Postoperative Care
- Euthanasia and Sample Extraction
- Micro-CT
- Statistics
- Histology
- Results
- Identification of Micropores of 60.66 24.48 m Diameter in the Clinically Approved Collagen Sponge Spongostan
- Spongostan Reveals a Distinct Nanotopography of 32.97 1.41 nm Pores
- Adult Human Stem Cells Efficiently Undergo Osteogenic Differentiation by the Micro- and Nanotopography Present on Spongostan
- The Micro- and Nanotopography of Spongostan is Solely Sufficient to Completely Regenerate a Critical-size Calvarial Rat Bone Defect
- Successful Masking of Micro- and Nanopores Using SiO2 Nanoparticles Impairs the Osteoinductive Properties of Spongostan In Vivo
- Discussion
- Conclusions
- References