Guerrero-Garzón, Jaime Felipe; Zehl, Martin; Schneider, Olha; Rückert, Christian ; Busche, Tobias; Kalinowski, Jörn; Bredholt, Harald; Zotchev, Sergey B.: Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products. In: Frontiers in Microbiology. Jg.11. 2020
Inhalt
- Sampling and Treatment of Sponge Samples
- Bacterial Strains and Growth Conditions
- Cultivation, Extracts Preparation, and Bioassays
- DNA Isolation, PCR, and Phylogenetic Analysis
- Genome Sequencing, Assembly, and Analyses
- Construction of the ADI95-16 Knock-Out Mutant
- Production, Purification, and Identification of Linearmycins and Echinomycins
- Results
- Isolation and Preliminary Characterization of Streptomyces spp. From the Marine Sponge Antho dichotoma
- Comparative Genomics of Sponge-Associated Streptomyces Focused on Secondary Metabolite Biosynthesis Gene Clusters
- Gene Clusters for Lantipeptide and Thiopeptide Biosynthesis
- Gene Clusters for Polycyclic Tetramate Macrolactam Biosynthesis
- Bioactivity of Some Sponge-Derived Streptomycetes Is Due to Production of Linearmycins, Cycloheximide, and Echinomycins
- Comparative Genomics of Marine Streptomyces sp. ADI95-16 and Terrestrial Streptomyces sp. Mg1
- Discussion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References