Ruiz-Perera, Lucia Mercedes; Greiner, Johannes ; Kaltschmidt, Christian; Kaltschmidt, Barbara: A Matter of Choice: Inhibition of c-Rel Shifts Neuronal to Oligodendroglial Fate in Human Stem Cells. In: Cells. Jg.9 H. 4. 2020
Inhalt
- Materials and Methods
- Isolation and Cultivation of Human NCSCs
- Neuronal Differentiation of Human NCSCs to Glutamatergic Neurons
- Pentoxifylline Treatment
- Cerebellar Slice Culture, Demyelination and Cell Transplantation
- Immunocytochemistry
- Quantitative Polymerase Chain Reaction (qPCR)
- Image Analyses and Quantification
- Statistical Analyses
- Results
- NF-B-c-REL during Early Stages of Glutamatergic Differentiation in NCSC-Derived NSCs
- NF-B-c-REL is Crucial for Cell Survival during Glutamatergic Differentiation of Adult Human Stem Cells
- c-REL Inhibition by Pentoxifylline Induces a Shift into the Oligodendrocyte Fate
- Transplanted PTXF-Treated Predifferentiated NSCs into Demyelinated Mouse Organotypic Cerebellar Slices Integrate and Differentiate into Myelinating Oligodendrocytes
- Discussion
- References