Saadouli, Ilhem; Zendah El Euch, Imene; Trabelsi, Emna; Mosbah, Amor; Redissi, Alaeddine; Ferjani, Raoudha; Fhoula, Imene; Cherif, Ameur; Sabatier, Jean-Marc; Sewald, Norbert ; Ouzari, Hadda-Imene: Isolation, Characterization and Chemical Synthesis of Large Spectrum Antimicrobial Cyclic Dipeptide (l-leu-l-pro) from Streptomyces misionensisV16R3Y1 [...]. In: Antibiotics (Basel, Switzerland). Jg.9 H. 5. 2020
Inhalt
- Introduction
- Materials and Methods
- Chemicals and Media
- Microbial Strains Origin
- Antimicrobial Activity Tests
- Minimum Inhibitory Concentration (MIC) Assay
- Screening of Solvent for the Extraction of Antimicrobial Compound V16R3Y1
- Purification of Bioactive Compounds
- Thin Layer Chromatography (TLC) Procedure
- Gas Chromatography–Mass Spectrometry (GC–MS) Analysis
- 1H Nuclear Magnetic Resonance (NMR)
- Application of Metabolomic Procedure for the Identification of the Extracted Metabolites
- Synthesis of the Cyclo Dipeptide (Leu-Pro) in Solution
- Liquid Chromatography–Mass Spectrometry (HPLC–MS) Analysis
- Results
- Extraction and purification of Bioactive Compounds
- Gas Chromatography–Mass Spectrometry (GC–MS) Analysis
- NMR Spectra Analysis with the New Procedure
- Cyclo Dipeptide Synthesis
- Biological Activity of Biological Fractionand Synthetic Peptide
- Discussion
- Conclusions
- References