Liebthal, Michael; Schütze, Johannes; Dreyer, Anna ; Mock, Hans-Peter; Dietz, Karl-Josef: Redox Conformation-Specific Protein–Protein Interactions of the 2-Cysteine Peroxiredoxin in Arabidopsis. In: Antioxidants. Jg.9 H. 6. 2020
Inhalt
- Materials and Methods
- Plant Growth and Extraction of Proteins
- Affinity-Based Interaction Assay
- Peptide Preparation and Mass Spectrometric Analysis
- Cloning and Heterologous Expression of Recombinant Proteins
- Isothermal Titration Microcalorimetry (ITC)
- Far Western Overlay
- LIPOXYGENASE and ß-CARBONIC ANHYDRASE Activity Assays
- Results
- Discussion
- 2-CysPRX Protein–Protein Interactions: Comparing Current Knowledge and New Results
- Lack of 2-CysPRX in Plants: A Focus on Transcript and Metabolite Level
- Novel Interaction Partners of 2-CysPRX and Redox Regulation
- Conclusions
- References