Inhalt

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   Contents
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   General Introduction and Theory
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   Introduction and Motivation
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  Microfluidics
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  Hydrodynamics
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   Navier-Stokes equation
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   Hagen-Poiseuille flow
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   Reynolds number
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   Stokes friction
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   Surface potential and the electrical double layer
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   Electrokinetics
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   Electrophoresis and electroosmosis
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   Dielectrophoresis
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   Brownian motion and diffusion
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  Optical Tweezers
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   History
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  Gaussian and laser optics
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   Free space Gaussian optics
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   Focused Gaussian optics
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   Real Gaussian beams
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   Laser
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   Fibre laser
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   Polarisation
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  Theory of optical trapping
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   2D optical traps
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   3D optical traps
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   Non-Gaussian beam profiles
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   Hooke's Law
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   Langevin equation
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   Force analysis
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   Scattered light analysis
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   Video-based analysis
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   Interference effects in the vicinity of surfaces
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  Force calibration
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   Stokes' Law
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   Power Spectral Density (PSD) analysis
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  Theory of Nanopore translocation
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  DNA
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   Structure and role
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   Entropic behaviour
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   Electrical resistance of nanopores
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  Theory of Cell Elasticity
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   Setup of a cell
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  Viscoelasticity
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   Stress and Strain
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   Viscoelastic models
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   Purely elastic behaviour (spring)
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   Purely viscous behaviour (dashpot)
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   Series and parallel behaviour
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   Multi-element models
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  Experimental determination of viscoelastic parameters
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   Creep recovery test
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   Stress relaxation test
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   Oscillatory analysis
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   General method
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   DNA translocation through nanopores in boron nitride and molybdenum disulfide
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  Experimental setup
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   Optical setup
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   Microfluidic setup
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   Software
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   Monolayer membrane and nanopore preparation
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  Boron nitride
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   Material properties
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   Exfoliation
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   Transfer to substrate
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   Monolayer identification
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   Transfer to membrane
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   Molybdenum disulphide
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   Material properties
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   Exfoliation
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   Transfer to substrate
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   Monolayer identification
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   Transfer to membrane
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   Nanopore milling
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  Results
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   High-speed force detection
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  Boron nitride
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   Silicon wafer substrate
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   PDMS substrate
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   PMMA substrate
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   Molybdenum disulphide
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   Identification of monolayer flakes on PDMS
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   Wedging transfer
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   Transfer by viscoelastic stamping
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   Nanopore milling
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   DNA translocation with optical tweezers
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   Free DNA translocation
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   Conclusions and Outlook
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   Cell elasticity investigation with Elasto-Tweezers
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  Experimental setup
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   Optical tweezers setup
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   Microfluidics setup
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   General setup
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   Microfluidic chip
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   Temperature control
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   Software
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  Experimental protocols
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   Cell culture
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  Manufacture of microfluidic chips
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   PDMS chips
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   COC chips
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   Bead surface modifications
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   Declogging of microfluidic switches
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   Preparation of solutions
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   Installation and preparation of microfluidic chip
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   Cell viability verification
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  Results
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   Temperature control
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   Polarisation
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  Bead surface modifications
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   Carboxylated beads
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   Amino coated beads
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   Uncoated beads
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  Microfluidic design performance
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   Design 1: Corridors with adjacent trapping chambers
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   Design 2: Linear design
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   Design 3: Cross design with integrated experimental areas
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  Cell elasticity measurements
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   Cluster of HEK-293 cells and PDMS residue
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   Single HEK-293 cell with one static bead
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   Single HEK-293 cell with two optically trapped beads
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   Very stiff single HEK-293 cell
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   Cell viability verification
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   Conclusions and Outlook
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   Conclusions, Outlook and Appendix
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   Summary and Outlook
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  User manual for the nanopore setup
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   Safety information
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  System overview
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   Hardware overview
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   Software overview
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  Performing experiments
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   Turning the system on
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   Controlling andor.vi
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   Controlling tweezer2.vi
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   Packing data
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  Analysing data
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   Measurement selection
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   Frame Selection & Edge Detection Settings
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   Autofocus calibration
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   Force Analysis
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   Results
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  User manual for the Elasto-Tweezers setup
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   Safety information
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  System overview
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   Hardware overview
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   Software overview
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  Performing experiments
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   Turning the system on
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   Controlling ET.vi
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   Controlling overview.vi
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   Resolving common issues
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   Packing data
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   Force analysis
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   Measurement selection
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   Frame Selection & Edge Detection Settings
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   Force Analysis
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   Results
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   Stress / strain analysis
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   Fitting numerical solutions of first-order differential equations with Gnuplot
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   List of figures
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   List of publications and conference contributions
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   Bibliography
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   Danksagung