Rettenmaier, Regina; Schneider, Martina; Munk, Bernhard; Lebuhn, Michael; Jünemann, Sebastian; Sczyrba, Alexander ; Maus, Irena; Zverlov, Vladimir; Liebl, Wolfgang: Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. In: Microorganisms. Jg.8 H. 6. 2020
Inhalt
- Materials and Methods
- Data Availability
- Reactor Operation and Sampling
- Organisms
- Growth Conditions Used for D. raffinosedens 249c-K6 and H. thermocellum DSM 1237T
- Determination of Cellulose Hydrolyzing Activities by the Anthrone Assay of H. thermocellum Alone or in Co-Culture with D. raffinosedens
- Determination of Volatile Acids and Alcohols with Gas Chromatography
- Enzymatic Digestion of Cellulose Biomass and Determination of Enzymatic Activity
- Species Identification, Sequencing, and Annotation of D. raffinosedens, Isolate 249c-K6
- Prevalence and Activity of D. raffinosedens in Mesophilic and Thermophilic Biogas Fermenters Applying 16S rRNA Gene Amplicon Sequencing
- Occurrence of D. raffinosedens Sequences in Biogas-Producing Microbial Communities Deduced from 16S rDNA and Reverse Transcribed 16S rRNA Amplicons of this Study or from Publicly Available Metagenome Data
- Results and Discussion
- Isolation of D. raffinosedens from Cellulolytic Mixed Cultures Isolated from Biogas Fermenters
- Genome Sequence Analysis of the Strain D. raffinosedens 249c-K6
- Phylogenetic Classification of D. raffinosedens 249c-K6 in Relation to Members of the Genus Defluviitalea
- D. raffinosedens 249c-K6 Metabolic Pathways Predicted by Means of KEGG
- D. raffinosedens 249c-K6 Genes Predicted to be Involved in Carbohydrate Utilization
- Physiological Comparison of H. thermocellum as Pure Culture and in Co-Culture with D. raffinosedens
- Importance of the Genus Defluviitalea in Microbial Communities of Biogas Fermenters
- Conclusions
- References