Delépine, Baudoin; Gil Lopez, Marina; Carnicer, Marc; Vicente, Claúdia; Wendisch, Volker F. ; Heux, Stéphanie: Charting the metabolic landscape of the facultative methylotroph Bacillus methanolicus. In: mSystems. Jg.5. 2020
Inhalt
- In vitro assessment of arabitol assimilation.
- 13C-MFA experimental design.
- Measurement of physiological parameters.
- In vivo characterization of arabitol assimilation.
- In vivo operation of the pentose phosphate pathway with the different carbon sources.
- In vivo operation of the TCA cycle with the different carbon sources.
- Analysis of cofactor usage with the various carbon sources.
- Conclusion.
- MATERIALS AND METHODS
- Strain.
- Nonstationary 13C fluxomics experiment.
- Stationary 13C fluxomics experiments.
- Models and simulations.
- Analysis of arabitol phosphate dehydrogenases AtlD and AtlF.
- Data availability.
- SUPPLEMENTAL MATERIAL
- ACKNOWLEDGMENTS
- REFERENCES