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Kumar, Vijay; Vogelsang, Lara; Schmidt, Romy; Sharma, Shanti S.; Seidel, Thorsten; Dietz, Karl-Josef: Remodeling of Root Growth Under Combined Arsenic and Hypoxia Stress Is Linked to Nutrient Deprivation. In: Frontiers in Plant Science. Jg.11. 2020
Inhalt
Materials and Methods
Plant Growth and Stress Application
Microarray and Quantitative Transcript Analysis
Root Growth Phenotype and Confocal Imaging
Root Hair Phenotype
Cell-Viability Analysis
Fluorescence Analysis for Reactive Oxygen Species (ROS)
Biochemical Analysis
Ferric Chelate Reductase Activity
Activities of Hypoxia-Responsive Enzymes of Energy Metabolism
XTT Reductase Activity
Activities of Antioxidant Enzymes
Plant Cell Sap Osmolarity
Statistical Analysis
Results
Root Growth
Transcriptional Regulation of Root Architecture
Phosphate Starvation Response
Cell Viability Analysis in the Root Hair Zone
Iron Assimilation (and NO Signaling)
Arsenic Interference With Hypoxia Response in Root Stress Adaptation
Redox Regulation of Root Growth
Discussion
Suppression of Root Growth With Concomitant Stimulation of Root Hair Development
Interaction of Nutrient Homeostasis Networks
Phosphate Starvation
Fe-Homeostasis and Interaction With NO
Redox Regulation of Root and Root Hair Growth Under HpxAs
Transcripts Associated With Remodeling of Root Architecture Under HpxAs
Stem Cell Management
Energy Constraints and Stress Acclimation
Epidermal Cell Differentiation and Root Hair Development
Conclusion
Data Availability Statement
Author Contributions
Funding
Acknowledgments
Supplementary Material
References