Prell, Carina; Burgardt, Arthur; Meyer, Florian ; Wendisch, Volker F.: Fermentative production of ʟ-2-hydroxyglutarate by engineered Corynebacterium glutamicum via pathway extension of ʟ-lysine biosynthesis. In: Frontiers in Bioengineering and Biotechnology. Jg.8. 2021
Inhalt
- Microorganisms and Cultivation Conditions
- Molecular Biology Methods
- Coupled in vitro Activity of GabT and GabD
- Quantification of Amino Acids, Diamines, and Carboxylic Acids
- Fermentative Production
- Results
- Establishing de novo Biosynthesis of L-2HG by C. glutamicum
- Role of the Succinate Exporter SucE in Export of Glutarate and L-2HG
- Enhanced Conversion of Glutarate to L-2HG by Increased Iron Concentrations
- Inhibitory Effects on Key Enzymes in L-2HG Pathway
- L-2HG Production at Bioreactor Scale
- L-2HG Production From Wheat Sidestream Concentrate
- Discussion
- Data Availability Statement
- Author's Note
- Author Contributions
- Funding
- Acknowledgments
- References