Castellani, Lucas G.; Luchetti, Abril; Nilsson, Juliet F.; Pérez-Giménez, Julieta; Wegener, Caren; Schlüter, Andreas ; Pühler, Alfred ; Lagares, Antonio; Brom, Susana; Pistorio, Mariano; Niehaus, Karsten; Torres Tejerizo, Gonzalo A.: Exopolysaccharide Characterization of Rhizobium favelukesii LPU83 and Its Role in the Symbiosis With Alfalfa. In: Frontiers in Plant Science. Jg.12. 2021
Inhalt
- Bacterial Strains and Plasmids
- EPS and Total Proteins Quantification
- EPS Composition and Structure
- Bacterial Matings
- DNA Manipulation and Genetic Constructs
- Bioinformatics Approaches and Phylogenetic Analyses
- Plant Assays
- Results and Discussion
- Localization and Gene Arrangement of Exopolysaccharide-Related Genes in R. favelukesii LPU83
- Capsular Polysaccharide
- Galactoglucan
- Succinoglycan
- Arabinose-Containing Polysaccharide
- Cyclic -Glucans (CG) and Mixed-Linkage -Glucans (MLG)
- Phylogenetic Analyses of EPS I Genes in Rhizobium favelukesii LPU83
- The Exopolysaccharide Produced by R. favelukesii LPU83 Is Similar to the Succinoglycan of E. meliloti
- Genomic Regions Required for EPS Production of LPU83
- Symbiotic Phenotype of the LPU83 EPS Mutants
- Concluding Remarks
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References