Sasikumar, Keerthi; Hannibal, Silvin; Wendisch, Volker F. ; Nampoothiri, Madhavan: Production of biopolyamide precursors 5-amino valeric acid and putrescine from rice straw hydrolysate by engineered Corynebacterium glutamicum. In: Frontiers in Bioengineering and Biotechnology. Jg.9. 2021
Inhalt
- Bacterial Strains and Culture Conditions
- Genetic Manipulations
- Molecular Design and Genetic Engineering of C. glutamicum PUT Xyl and AVA Xyl
- Quantification of Sugars and Amines in the Fermented Broth
- Authentication of Putrescine Production From Xylose by Engineered C. glutamicum PUT Xyl
- Simultaneous Utilization of Glucose and Xylose by the Engineered C. glutamicum Strains in CGXII Minimal Medium by Batch Fermentation
- Preparation of Rice Straw Hydrolysate (RSH)
- Utilization of RSH as a Sole Carbon Source by AVA Xyl and PUT Xyl
- Results and Discussion
- Validation of Biosynthesis of Putrescine by the Recombinant Strain PUT Xyl in CGXII Medium
- Production of Putrescine and 5-AVA by PUT Xyl and AVA Xyl in CGXII Minimal Medium With the Simultaneous Utilization of Glucose and Xylose
- Exploitation of the Fermentable Sugars in the RSH for the Production of Putrescine and 5-AVA
- Conclusion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References