Maynard, Daniel; Viehhauser, Andrea; Knieper, Madita; Dreyer, Anna ; Manea, Ghamdan; Telman, Wilena; Butter, Falk; Chibani, Kamel; Scheibe, Renate; Dietz, Karl-Josef: The In Vitro Interaction of 12-Oxophytodienoic Acid and Related Conjugated Carbonyl Compounds with Thiol Antioxidants. In: Biomolecules. Jg.11 H. 3. 2021
Inhalt
- Materials and Methods
- Chemicals
- Data Processing, Databases and in Silico Studies
- Synthesis and Detection of Cys-12-OPDA
- Mass Spectrometric and Calorimetric Detection of Cysteine Interacting with Abscisic Acid
- Analysis of Free Thiol Content of GSH Incubated with CCCs
- Effect of Exogenously Applied Methylvinyl Ketone (MVK) Incubated with Thiol and Non-thiol Antioxidant on Photochemical Activity of Leaf Discs
- Synthesis and Purification of FBPase, TRX-f1, TRX-m1, 2-CysPRX, NTRC, Cyp20-3 and Cyp20-3 Variants
- Fluorescence Properties of TRX-Fold Proteins and Cyp20-3
- Covalent Modification of TRX-f1 by 12-OPDA for Analysis by Mass Spectrometry
- FBPase Activity Test
- Protein Thiol Estimation with DTNB
- NTRC-Dependent Peroxidase Activity of 2-CysPRX in Presence of CCCs
- ITC-Analysis of Interaction between 12-OPDA and Cyp20-3
- Activity Assays for Cyp20-3 in the Presence of 12-OPDA
- Results
- Reactivity of Michael Systems with Cysteine, Glutathione and Ascorbate
- The Interaction of 12-OPDA with Plant Thioredoxin
- The Interaction of 12-OPDA with Cyclophilin 20-3
- Discussion
- Conclusions
- References