Kugler, Pierre; Trumm, Marika; Frese, Marcel ; Wendisch, Volker F.: L-Carnitine production through biosensor-guided construction of the Neurospora crassa biosynthesis pathway in Escherichia coli. In: Frontiers in Bioengineering and Biotechnology. Jg.9. 2021
Inhalt
- Bacterial Strains, Recombinant DNA Work, Media, and Growth Conditions
- Biosensor Experiments
- Assay of TMABA Dehydrogenase Activity in E. coli Crude Extracts
- Protein Gel Analysis
- Carnitine Production Experiment With Recombinant E. coli Expressing the Carnitine Biosynthetic Pathway From N. crassa
- LC-MS Measurement
- Analytical Quantification of Glycerol in the Carnitine Production Experiment
- Reagents
- Results
- Selection and Cloning of the l-Carnitine Biosynthetic Pathway Genes From Neurospora crassa
- Screening for Functional γ-Butyrobetaine Hydroxylases
- Screening for Functional TMABA Dehydrogenases
- Assembly of the l-Carnitine Biosynthetic Pathway
- Improving the l-Carnitine Biosynthesis Pathway for de novo Production by E. coli
- LC-MS Analysis of l-Carnitine Production by Recombinant E. coli Expressing the l-Carnitine Biosynthetic Pathway from N. crassa
- Discussion
- Data Availability Statement
- Author Contributions
- Funding
- Supplementary Material
- References