Wördemann, Ramona; Wiefel, Lars; Wendisch, Volker F. ; Steinbüchel, Alexander: Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum. In: AMB Express. Jg.11 H. 1. 2021
Inhalt
- Bacterial strains, media, and growth conditions
- Cultivation of C. glutamicum strain DM1729 pVWEx1::cphA6308 Δ1_C595S in a stirred tank reactor
- Molecular biology techniques
- Isolation of cyanophycin from C. glutamicum
- Analysis of isolated cyanophycin
- Results
- Cyanophycin synthesis in C. glutamicum ATCC 13032
- Cyanophycin synthesis in a C. glutamicum mutant for lysine production
- Cyanophycin synthesis in a C. glutamicum mutant for ornithine production
- Cyanophycin synthesis in a C. glutamicum mutant for cadaverine production
- Cultivation in stirred tank reactors and scale up
- Discussion
- References