Prell, Carina; Busche, Tobias; Rückert, Christian ; Nolte, Lea; Brandenbusch, Christoph; Wendisch, Volker F.: Adaptive Laboratory Evolution accelerated glutarate production by Corynebacterium glutamicum. In: Microbial Cell Factories. Jg.20. 2021, S. 97 ff.
Inhalt
- Results
- Adaptive laboratory evolution of flux enforced glutarate production improved volumetric productivity by metabolically engineered C. glutamicum
- RNAseq analysis of global gene expression patterns in ALE strains GluA T5 and GluA T7
- Genome sequencing and reverse genetics for identification of mutations causal for improved glutarate production by ALE strains GluA T5 and GluA T7
- The beneficial effect of GltBE686Q depends on the deletion of gdh
- Fermentative glutarate production by evolved strain GluA T7 in comparison to GluA T0
- Reactive extraction of glutarate from the cultivation broth
- Discussion
- Material and methods
- Microorganisms and cultivation conditions
- ALE experiments
- Molecular biology methods
- Quantification of amino acids, carbohydrates and organic acids by HPLC
- Coupled in vitro activity of GabT and GabD
- In vitro activity of GltBD
- Fermentative production
- Whole-genome sequencing
- RNA isolation, cDNA library preparation and sequencing
- Reactive extraction of glutarate
- Acknowledgements
- References