Theine, Jens; Holtgräwe, Daniela ; Herzog, Katja; Schwander, Florian; Kicherer, Anna; Hausmann, Ludger; Viehöver, Prisca; Töpfer, Reinhard; Weisshaar, Bernd: Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling [...]. In: BMC Plant Biology. Jg.21. 2021
Inhalt
- Results
- Phenotypical comparison between two Pinot cultivars
- Global view of gene expression patterns
- Cluster analysis for identification of co-expressed genes
- Analyses of differentially expressed genes
- Functional classification of DEGs
- Putative candidates for ripening time control genes
- Discussion
- Phenotypic differences between the cultivars PN and PNP
- General validation of the RNA-Seq dataset
- Co-expression analysis shows similar gene expression clusters between cultivars and years
- Differentially expressed genes throughout berry development and identification of veraison-specific genes
- Putative ripening time control genes acting early in berry development
- Candidates for causal genes explaining the difference between PN and PNP
- Conclusions
- Material and methods
- Plant material and analysis of clonal relation
- Phenotypical characterization and sampling of plant material
- RNA extraction and cDNA library construction
- RNA-Seq
- Processing of RNA-Seq read data
- Basic gene expression analyses
- Principal component analysis
- Functional annotation of genes
- Cluster analysis
- Differential gene expression analyses
- Confirmation of differential gene expression by qRT-PCR
- Selection of gene sets potentially relevant for ripening time and comparison with literature data
- Visualization of gene numbers newly appearing as differentially expressed
- Pathway enrichment analysis
- Heatmaps
- Acknowledgements
- References