Schmitz, Julian ; Hertel, Oliver ; Yermakov, Boris; Noll, Thomas ; Grünberger, Alexander: Growth and eGFP-production of CHO-K1 suspension cells cultivated from single-cell to laboratory scale. In: Frontiers in Bioengineering and Biotechnology. Jg.9. 2021
Inhalt
- Cell Culture and Medium
- Microfluidic Single-Cell Cultivation
- Shake Flask Cultivation
- Bioreactor Cultivation
- Growth Rate Analysis
- Cell Morphology Analysis
- Fluorescence Analysis
- Results and Discussion
- Key Characteristics of Stirred Tank Bioreactor, Shake Flask, and MSCC
- Comparison of Growth Behavior
- Comparison of Cellular Morphology
- Green Fluorescent Protein Production
- Conclusion and Outlook
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References