Busche, Mareike ; Acatay, Christopher; Martens, Stefan; Weisshaar, Bernd ; Stracke, Ralf: Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis. In: Frontiers in Plant Science. Jg.12. 2021
Inhalt
- Materials and Methods
- Plant Material
- Phylogenetic Analysis
- Expression Analysis
- Total RNA Extraction, cDNA Synthesis and Molecular Cloning
- Heterologous Expression in E. coli
- F3H and FLS Bioconversion Assay in E. coli
- Agrobacterium -Mediated Transformation of A. thaliana
- Flavonoid Staining of A. thaliana Seedlings
- Analysis of Flavonols in Methanolic Extracts
- Determination of Anthocyanin Content
- Results
- Creation of cDNA Constructs and Sequence-Based Characterisation of Musa Flavonoid 2-ODDs
- Expression Profiles of Musa 2-ODD Genes
- Musa F3H1 and Musa F3H2 Are Functional F3H
- Musa FLS1 and Musa FLS3 Are Functional FLS
- Musa ANS Is a Functional ANS
- Discussion
- Musa F3H1 and Musa F3H2
- Musa FLS1 and Musa FLS3
- Musa ANS
- Data Availability Statement
- Author Contributions
- Supplementary Material
- References