Geiger, Otto; Sohlenkamp, Christian; Vera-Cruz, Diana; Medeot, Daniela B; Martinez-Aguilar, Lourdes; Sahonero-Canavesi, Diana X; Weidner, Stefan; Pühler, Alfred ; Lopez-Lara, Isabel M: ExoS/ChvI Two-Component Signal-Transduction System Activated in the Absence of Bacterial Phosphatidylcholine. In: Frontiers in Plant Science. Jg.12. 2021
Inhalt
- PC-Deficient Mutant of S. meliloti Cannot Form Nitrogen-Fixing Root Nodules on Alfalfa
- Growth Analysis of S. meliloti Wild Type and Phospholipid-Deficient Mutants
- Transcriptional and Physiological Differences Between Wild Type and PE-Deficient Mutant Cells
- Transcriptional Differences Between Wild Type and PC-Deficient Mutant Cells
- PC-Deficient Mutant Is Impaired for Swimming and Overproduces Succinoglycan
- PC-Deficient Mutant Is Impaired for Growth at Slightly Acidic pH
- Some Mutants Altered in the ExoR/ExoS/ChvI Signal Transduction System Resemble the PC-Deficient Mutant
- Isolation of PC-Deficient Suppressor Mutants That Regain Capacity to Swim and Produce Less Succinoglycan
- S. meliloti Suppressor Mutants M16 and M22 Carry Reduced Function ExoS Mutations
- ExoR Levels Are Similar in Wild Type, PC-Deficient Mutant and Suppressor Mutants
- Discussion
- Model for the Regulation of the ExoR/ExoS/ChvI Signal Transduction System
- ExoR/ExoS/ChvI Orthologs and PC Co-occur in the Rhizobiales
- Diverse Roles for PC in Bacteria
- Materials and Methods
- Bacterial Strains, Media and Growth Conditions
- Detection of Lipochitin Oligosaccharide Nodulation Factors by Thin-Layer Chromatography
- Transcriptional Profiling Using the SM14kOligo Whole Genome Microarray
- Reverse Transcription Quantitative Real-Time PCR
- Motility Assays
- Exopolysaccharide Production
- DNA Manipulations
- Cloning and Expression of exoS and exoR Genes From S. meliloti
- Insertion of a Gentamicin Resistance-Conferring Cassette Into the Genome of S. meliloti at a Distance of 15 kb From exoS
- In vivo Labeling of Sinorhizobium meliloti With [14C]Acetate and Quantitative Analysis of Lipid Extracts
- Preparation of ExoR Antibodies and Their Purification
- Detection of Different Forms of ExoR by Western Blot Analysis
- Plant Assays
- Statistical Analysis
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References