Täuber, Sarah ; Schmitz, Julian ; Blöbaum, Luisa; Fante, Niklas; Steinhoff, Heiko; Grünberger, Alexander: How to Perform a Microfluidic Cultivation Experiment—A Guideline to Success. In: Biosensors. Jg.11 H. 12. 2021
Inhalt
- Experimental Workflow
- Microfluidic Design and Fabrication
- PDMS Chip Assembly
- Cell and Medium Preparation
- Hardware Preparation
- Device Loading
- Cultivation
- Live-Cell Imaging
- Challenges during a MC Experiment
- Fabrication Errors
- Biological Contamination
- Inefficient Chip Loading
- Leaking Periphery
- Air Bubbles during Cultivation
- Compromised Flow
- Loss of Analysed Cells
- Undesired Growth Locations
- Unexpected Growth Behaviour
- Blurry Images
- Focus Problems
- Premature Experimental Termination
- Case Studies
- Case Study I—Establishing MSCC for a New Organism
- Case Study II—Getting Cells to Grow Reproducibly
- Case Study III—Growing Cells without Carbon Source
- Conclusions
- References