Prell, Carina; Vonderbank, Sophie; Meyer, Florian ; Perez, Fernando; Wendisch, Volker F.: Metabolic engineering of Corynebacterium glutamicum for de novo production of 3-hydroxycadaverine. In: Current Research in Biotechnology. Jg.4. 2022, S. 32-46
Inhalt
- Material and methods
- Microorganisms and cultivation conditions
- Molecular biology methods
- Quantification of amino acids, carbohydrates and organic acids by HPLC
- Determination of substrate specificity via biotransformation
- Fermentative production of 3-HC
- Results
- Determination of the substrate spectra of the lysine-4-hydroxylase KDOFj
- Determination of the substrate specificity of different lysine decarboxylases
- Fermentative production of hydroxylated lysines via regiospecific C-H-hydroxylation using different KDOs
- Role of LysE for 4-hydroxylysine production
- Effect of increased supply of 2-oxoglutarate as cosubstrate on 4-HL production
- Effect of increased supply of iron as cofactor on 4-HL production
- Adaption of extracellular pH for improved precursor supply and 4-HL production
- Extension of the pathway from 4-HL to 3-HC
- Fermentative production of 3-HC in reactor scale
- Discussion
- Funding
- Declaration of Competing Interest
- Acknowledgments
- Appendix
- Ethical approval
- Appendix A Supplementary material
- References