Linder, Marten; Haak, Markus ; Botes , Angela; Kalinowski, Jörn; Rückert, Christian: Construction of an IS-Free Corynebacterium glutamicum ATCC 13 032 Chassis Strain and Random Mutagenesis Using the Endogenous ISCg1 Transposase. In: Frontiers in Bioengineering and Biotechnology. Jg.9. 2021
Inhalt
- 2 Materials and Methods
- 2.1 Bacterial Strains, Plasmids and Culture Media
- 2.2 DNA Isolation, Transfer and Manipulation
- 2.3 Construction of Plasmids
- 2.4 Site-specific Gene Disruption/Replacement
- 2.5 Transposon Mutant Sequencing
- 2.6 Determination of Growth Rates and Transformation Efficiency
- 2.7 Genome Sequencing, Assembly, and Accession Numbers
- 3 Results
- 3.1 Construction of Strain CR101
- 3.2 Development of a Genetic Switch Based System for Random Insertion Mutagenesis
- 3.3 Sequencing of ML103 Transposon Mutants
- 4 Discussion
- Data Availability Statement
- Author Contributions
- Acknowledgments
- Supplementary Material
- References