Luenenschloss, Andrea; ter Veld, Frank; Albaum, Stefan ; Neddermann, Tobias; Wendisch, Volker F. ; Poetsch, Ansgar: Functional genomics uncovers pleiotropic role of rhomboids in Corynebacterium glutamicum. In: Frontiers in Microbiology. Jg.13. 2022
Inhalt
- Experimental Design
- Construction of the Rhomboid Double Deletion Mutant
- Cultivation of C. glutamicum and Heat Shock Experiments
- DNA Microarray Analysis
- Antibody Generation and Detection of Cg2767
- Subcellular Fractionation and Protein Preparation
- Mass Spectrometry
- Protein Identification and Quantification
- Shotgun Lipidomics
- Results
- Construction of Δcg2767/Δcg0049 Strain and Characterization of Its Growth Phenotype
- Cg2767 Abundance Increases During Heat Stress
- Δcg2767/Δcg0049 Proteome Analysis, General Results
- Transcriptomics of Δcg2767/Δcg0049 in the Exponential Growth Phase at 30 and 40C
- Δcg2767/Δcg0049 Proteome Dynamics in the Stationary Growth Phase at 30 and 40˚C
- Energy Production and Conversion
- Cell Cycle Control, Cell Division, Chromosome Partitioning
- Amino Acid Transport and Metabolism
- Nucleotide Transport and Metabolism
- Carbohydrate Transport and Metabolism
- Coenzyme Transport and Metabolism
- Translation, Ribosomal Structure and Biogenesis
- Transcription
- Posttranslational Modification, Protein Turnover, Chaperones
- Inorganic Ion Transport and Metabolism
- Signal Transduction Mechanisms
- Intracellular Trafficking, Secretion, and Vesicular Transport
- Cell Wall/Membrane/Envelope Biogenesis
- Lipid Transport and Metabolism
- Comparative Shotgun Lipidomics of Δcg2767/Δcg0049 in the Stationary Growth Phase at 30 and 40C
- Discussion
- Consequences of Rhomboid Gene Deletion
- Putative Involvement of Cg2767 in Heat Stress Adaptation
- Putative Involvement of Rhomboids in Lipid Homeostasis
- Function in Respiratory Chain Protein Quality Control
- Rhomboid Function in Pathogenic Relatives
- Conclusion
- Data Availability Statement
- Author Contributions
- Acknowledgments
- Supplementary Material
- References