Willenbücher, Katharina; Wibberg, Daniel; Huang, Liren; Conrady, Marius; Ramm, Patrice; Gätcke, Julia; Busche, Tobias; Brandt, Christian; Szewzyk, Ulrich; Schlüter, Andreas ; Barrero Canosa, Jimena; Maus, Irena: Phage Genome Diversity in a Biogas-Producing Microbiome Analyzed by Illumina and Nanopore GridION Sequencing. In: Microorganisms. Jg.10 H. 2. 2022
Inhalt
- 2. Materials and Methods
- 2.1. Reactor Set-Up and Sampling
- 2.2. Electron Microscopy
- 2.3. Enrichment and Purification of Phage Particles from a Biogas Microbiome
- 2.4. Phage DNA Extraction
- 2.5. MinION Library Preparation and Sequencing
- 2.6. Illumina Library Preparation and Sequencing
- 2.7. Base Calling, Read Processing, and Assembly
- 2.8. Virome Filtering, Annotation, and Genome Analysis
- 2.9. Fragment Recruitment
- 3. Results and Discussion
- 3.1. Occurrence of Virus-like Particles in the Studied Biogas Reactor by Means of Transmission Electron Microscopy
- 3.2. Virome Separation, Enrichment, Sequencing, and Bioinformatic Analysis
- 3.3. Phage Diversity in the Studied Biogas Plant as Analyzed by Means of Viral Metagenome Data
- 3.4. Protein-Based Phage Similarity Network Showed Relationships with Phages Infecting Members of the Classes Bacilli and Clostridia
- 3.5. Insights into the Biogas Plant Phages Life Cycle by Comprehensive Genome Analysis
- 3.6. Occurrence of Phage Relatives in Biogas-Producing Microbial Communities as Deduced from Publicly Available Metagenome Data
- 4. Conclusions
- References