TY  - BOOK
AB  - Native hyaline cartilage from a human knee joint was directly investigated with laser scanning microscopy via 2-photon autofluorescence excitation with no additional staining or labelling protocols in a nondestructive and sterile manner. Using a femtosecond, near-infrared (NIR) Ti:Sa laser for 2-photon excitation and a dedicated NIR long distance objective, autofluorescence imaging and measurements of the extracellular matrix (ECM) tissue with incorporated chondrocytes were possible with a penetration depth of up to 460 µm inside the sample. Via spectral autofluorescence separation these experiments allowed the discrimination of chondrocytes from the ECM and therefore an estimate of chondrocytic cell density within the cartilage tissue to approximately 0.2-2·10 7/cm³. Furthermore, a comparison of the relative autofluorescence signals between nonarthritic and arthritic cartilage tissue exhibited distinct differences in tissue morphology. As these morphological findings are in keeping with the macroscopic diagnosis, our measurement has the potential of being used in future diagnostic applications.
DA  - 2005
DO  - 10.1117/12.632940
KW  - Biomedical imaging
KW  - Multiphoton processes
KW  - Human cartilage
KW  - Three-dimensional microscopy
LA  - eng
PY  - 2005
SE  - 16-21
T2  - Proceedings of SPIE - The International Society for Optical Engineering
TI  - 2-Photon Laser Scanning Microscopy on Native Human Cartilage
UR  - https://nbn-resolving.org/urn:nbn:de:0070-bipr-21
Y2  - 2024-11-22T10:11:14
ER  -