TY  - JOUR
AB  - A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (< 3 µm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that [lambda]-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.
DA  - 2003
DO  - 10.1016/S0167-9317(03)00153-9
KW  - Fluorescence microscopy
KW  - Single molecule
KW  - Contact lithography
KW  - Microchip
KW  - Electrophoresis
KW  - DNA
LA  - eng
IS  - 1
M2  - 905
PY  - 2003
SN  - 0167-9317
SP  - 905-912
T2  - Microelectronic Engineering
TI  - Size-dependent free solution DNA electrophoresis in structured microfluidic systems
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17731753
Y2  - 2024-11-22T06:13:22
ER  -