TY  - JOUR
AB  - Visualization of proteins inside acrylamide and other gels usually relies on different staining methods. To omit the protein-staining procedure, we visualized unstained proteins inside acrylamide gels by laser excitation with ultraviolet (UV) light (280 nm, 35 mJ/cm²) and directly detected native UV fluorescence. In one-dimensional gels, a detection limit as low as 1 ng for bovine serum albumin and 5 ng for other proteins with a linear dynamic range (2.7 orders of magnitude) comparable to state of the art fluorescent dyes could be achieved. In addition, the application of this method to 20 µg of a whole cell lysate separated in a two-dimensional gel showed more than 600 spots. Since protein labeling always represents a serious obstacle in protein identification technologies, the working efficiency with our procedure can be considered as a significant improvement for protein visualization and reproducibility in proteomics.
DA  - 2003
DO  - 10.1021/ac020517o
LA  - eng
IS  - 1
M2  - 157
PY  - 2003
SN  - 0003-2700
SP  - 157-159
T2  - Analytical chemistry
TI  - Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17731903
Y2  - 2024-11-22T04:28:20
ER  -