TY  - JOUR
AB  - We applied two-photon laser-scanning microscopy (TPLSM) to motion-sensitive visual interneurons of the fly to study Ca(2+) dynamics in vivo at a higher spatial and temporal resolution than possible with conventional fluorescence microscopy. Based on a custom-built two-photon microscope, we performed line scans to measure changes in presynaptic Ca(2+) concentrations elicited by visual stimulation. We used a fast avalanche photodiode (APD) with a high quantum efficiency to detect even low levels of emitted fluorescence. Our experiments show that our in vivo preparation is amenable to TPLSM: with excitation intensities low enough not to cause photodamage, activity-dependent fluorescence changes of Ca(2+)-sensitive dyes can be detected in small neuronal branches. The performance of two-photon and conventional Ca(2+) imaging carried out consecutively at the same neuron is compared and it is demonstrated that two-photon imaging allows us to detect differences in Ca(2+) dynamics between individual neurites.
DA  - 2004
DO  - 10.1016/j.bbrc.2004.02.047
KW  - Fly
KW  - Two-photon microscopy
KW  - Tangential cell
KW  - Motion vision
KW  - fluo-4
KW  - Fluorescence imaging
KW  - Synapse
KW  - Calcium
KW  - Neuronal processing
LA  - eng
IS  - 2
M2  - 341
PY  - 2004
SN  - 0006-291X
SP  - 341-347
T2  - Biochemical and biophysical research communications
TI  - In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17734642
Y2  - 2024-11-22T03:09:51
ER  -