TY  - JOUR
AB  - The completion of the Human Genome Project provides researchers with a reference sequence that covers about 99% of the gene-containing regions and is more than 99.9% accurate. Sequence drafts and completed sequences for several other species are also available to researchers worldwide. The ongoing effort to provide more and more genomic reference information now enables the detection of deviations from this `genetic blueprint'. Comparative sequencing projects will play a major role in elucidating the meaning of the genetic code and in establishing a correlation between genotype and phenotype. As part of this effort, a number of projects will focus on distinct functional aspects, like resequencing of exons or HLA determining regions. Typically these target regions are short in length and their analysis does not require long read length. To find an efficient solution for these applications, we developed a novel method that allows simultaneous analysis of multiple independent target regions (Multiplexed Comparative Sequence Analysis) by employing base-specific cleavage biochemistry and MALDI TOF-MS analysis.
DA  - 2005
DO  - 10.1093/nar/gni038
LA  - eng
IS  - 4
M2  - e38
PY  - 2005
SN  - 1362-4962
SP  - e38-
T2  - Nucleic Acids Research
TI  - Multiplexed discovery of sequence polymorphisms using base-specific cleavage and MALDI-TOF MS
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17736908
Y2  - 2024-11-22T04:36:13
ER  -