TY  - JOUR
AB  - Polyelectrolyte field effects are indicated by particularly large variations of thermodynamic and kinetic constants with ionic strength. Some fundamental principles of local electric field effects in the microenvironment of polyelectrolyte structures are discussed, aiming at a reliable analysis of shifts in equilibrium and rate constants of ionic reaction partners with ionic strength. It is shown that the analytical expressions, within certain limitations, are suitable to determine effective charges involved in polyionic field effects on ionic reactions in the immediate neigborhood of a polyelectrolyte structure. An instructive example for such an observation is the neuro-enzyme acetylcholinesterase. The results of a relaxation-kinetic titration of this anionic enzyme (which hydrolyzes the cationic neuro-activator acetylcholine) with a cationic ligand suggest that the micro environment of the enzyme-active site consists of at least six anionic groups. A large effective, negative charge number is also reflected in the comparatively large association rate coefficients.
DA  - 1981
DO  - 10.1016/0302-4598(81)85010-6
KW  - Molecular biology
KW  - Biophysics
KW  - Biochemistry
LA  - eng
IS  - 1
M2  - 89
PY  - 1981
SN  - 0302-4598
SP  - 89-101
T2  - Bioelectrochemistry and Bioenergetics
TI  - Polyelectrolyte field effects
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17744556
Y2  - 2024-11-22T05:06:27
ER  -