TY  - JOUR
AB  - Measurement of tracer ion flux from or into a collection of closed membrane structures (CMS) constitutes a broadly applicable technique for studying ion channel gating by specialized gating molecules in biological membranes. The amplitudes for the flux process reflect the overall change in tracer content due to flux during a period in which channels on at least some of the CMS were open. In practice, the attainment of a time-invariant, finite overall tracer content, indicating a cessation of flux, need not imply that flux has reached completion, i.e., that the CMS internal and external tracer concentrations have fully reached equilibrium. Less than maximum flux amplitudes arise when binding of control ligands leads to an inhibition or inactivation of the channel gating molecules prior to a complete equilibration of tracer. Analysis of the dependence of the flux amplitudes on control ligand concentration permits determination of characteristic parameters of the CMS that may vary with the methods of preparation (e.g., the distributions of CMS size and CMS content of gating units).
DA  - 1982
DO  - 10.1016/0301-4622(82)80015-X
KW  - Tracer flux
KW  - Ion transport
KW  - Gating process
LA  - eng
IS  - 4
M2  - 317
PY  - 1982
SN  - 0301-4622
SP  - 317-325
T2  - Biophysical Chemistry
TI  - Single channel gating events in tracer flux experiments II. Flux amplitude analysis
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17744771
Y2  - 2024-11-22T04:41:08
ER  -