TY  - JOUR
AB  - After digestion of bacteriophage P1 DNA with EcoRI in the presence of P1 repressor, 6 repressor binding sites were identified in 5 of 26 EcoRI fragments. Binding sites were localized by the decreased mobility of DNA fragment-repressor complexes during electrophoresis and by DNase protection ("footprinting") analysis. The repressor binding sites, or operators, comprise a 17-base-pair-long consensus sequence lacking symmetrical elements. Three operators can be related to known genes, whereas the function of the others is still unknown. The mutant P1 bac, rendering ban expression constitutive, is identified as an operator-constitutive mutation of the ban operon.
DA  - 1987
KW  - operator sequence and mutation
KW  - repressor protein
KW  - DNA cloning
LA  - eng
IS  - 16
M2  - 5570
PY  - 1987
SP  - 5570-5574
T2  - Proceedings of the National Academy of Sciences of the United States of America
TI  - Multiple repressor binding sites in the genome of bacteriophage P1
UR  - https://nbn-resolving.org/urn:nbn:de:0070-bipr-12644
Y2  - 2024-11-22T06:09:51
ER  -