TY  - JOUR
AB  - Serum free fermentation procedures of cell cultures have got a wide application in production of biochemicals. But, cells cultured in serum free media in general are more sensitive to changes in culture condition, especially to nutrient limitation. There are no substances from serum which can support the cells when conditions are changing. In this study special attention is directed to amino acid utilization of mouse hybridoma in batch, chemostat and perfusion fermentations. Detailed data are presented which show the considerable difference of amino acid consumption rates in different fermentation modes. Already, in batch mode there are differences of the two investigated mouse hybridoma cell lines, although they are derived from the same myeloma line. In chemostat running at a dilution rate representing maximal growth rate most of the consumption rates are significant higher than in batch. On the other hand, in perfusion mode the rates are lower than in batch. This indicates clearly the different conditions of the fermentation modes. Therefore, it is necessary to develop serum free processes under the desired production conditions. An accurate analysis of the process is strongly recommended.
DA  - 1991
DO  - 10.1007/BF00365534
KW  - substrate utilization
KW  - serum-free medium
KW  - hybridoma
KW  - amino acid analysis
KW  - antibody production
LA  - ger
IS  - 1
M2  - 57
PY  - 1991
SN  - 0920-9069
SP  - 57-67
T2  - Cytotechnology
TI  - Optimization of serum-free fermentation processes for antibody production
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17823614
Y2  - 2024-11-22T04:18:18
ER  -