TY  - JOUR
AB  - Based on experiments in bench scale, a recycling of spent cell culture medium was performed in a 100-l pilot scale bioreactor. The cell cultivation has been done as a repeated batch procedure after the initial batch in the following four repeated batches spent medium from the previous batch was partially re-used. After microfiltration and ultrafiltration a part of the filtrate was mixed with a concentrate of amino acids and glucose, sterile filtered and subsequently filled back into the bioreactor. Up to 65% of the harvested cell- and product-free spent medium was re-used in each repeated batch. This procedure results in a saving of pure and waste water volume and saving of supplemented proteins as transferrin, insulin and lipoproteins and, therefore, also in a reduction of the production costs. A strongly acidic membrane ion exchanger was evaluated for the ability to purify the monoclonal antibodies from the pilot scale cultivation. Within minutes, gram quantities of product could be purified in a high flux system, especially developed for this purpose, achieving purities of 80%. The capacity of the acidic membrane ion exchanger was found in former investigations to be 1 mg cm -2 with recoveries up to 96%. Final purification was carried out by gel column filtration.
DA  - 1994
DO  - 10.1016/0168-1656(94)90060-4
KW  - membrane ion exchanger
KW  - medium
KW  - re-use
KW  - large scale
KW  - Monoclonal antibody
KW  - economy
LA  - eng
IS  - 3
M2  - 247
PY  - 1994
SN  - 0168-1656
SP  - 247-257
T2  - Journal of Biotechnology
TI  - Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17823991
Y2  - 2024-11-22T06:57:56
ER  -