TY  - JOUR
AB  - A DNA fragment from Bacillus subtilis strain Marburg coding for the synthesis of an enzyme catalyzing sucrose hydrolysis was cloned in Escherichia coli and detected using simple direct selection of transformants growing on sucrose. Three different clones were obtained each having a 2.5 kb EcoRI-Pstl fragment in common which was shown to be sufficient to mediate growth on sucrose. This fragment was not identical with known cloned gene fragments of B. subtilis coding for the sucrose hydrolyzing enzymes sucrase and levansucrase. It could be shown that the 2.5 kb fragment codes for a third sucrose hydrolyzing enzyme, namely for levanase. In the case of E. coli this enzyme was found to be mainly intracellular; however, a small quantity was also excreted into the periplasmic space.
DA  - 1986
DO  - 10.1016/0168-1656(86)90014-3
KW  - Escherichia coli
KW  - Bacillus subtilis
KW  - inulin; levanase
KW  - sucrose hydrolysis
KW  - recombinant DNA
LA  - eng
IS  - 5-6
M2  - 333
PY  - 1986
SN  - 0168-1656
SP  - 333-341
T2  - Journal of Biotechnology
TI  - Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-17825426
Y2  - 2024-11-21T22:51:37
ER  -