TY  - THES
AB  - To analyse molecular events in pathological processes and cell interactions underlying degenerative diseases, a genetic approach of conditional cell ablation is desirable. In my thesis, conditional cell ablation was optimized for applications in post-mitotic and slowly proliferative cells by modification of the tetracycline (tet)-dependent expression system in combination with the bacterial ribonuclease barnase. The regulated barnase expression and the cytotoxicity of the gene product were examined in cell culture, in primary cells of transgenic mice and in the nervous system of transgenic mice.
Taken together the analysis of this cell ablation approach demonstrates that in cell culture and to a limited extent in transgenic mice, a conditional cell ablation is feasible. By its universal application, e.g. by crossing barnase-transgenic mice with cell-specific transactivator mice, this system represents an alternative to existing methods for the investigation of pathomechanisms of degenerative diseases, also in the CNS.
DA  - 2003
KW  - , Tet-Expressionssystem , Konditionale Toxingenexpression , Neurodegeneration , Reaktive Astrogliose ,
LA  - ger
PY  - 2003
TI  - Entwicklung eines stringent kontrollierbaren Toxingensystems zur Analyse degenerativer Prozesse im Nervensystem der Maus
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:361-5101
Y2  - 2024-11-22T03:15:45
ER  -