TY  - THES
AB  - Chromatographic separations form the basis for the purification of proteins and are characterized by a complex interplay of adsorption and mass transport phenomena in porous stationary phases. Ion exchange chromatography is the most widely employed chromatographic process for the initial recovery and purification of proteins. Size exclusion chromatography separates proteins based on their size and is often used for the final polishing step of a protein. For both chromatographic methods optimal process conditions cannot be derived a priori from protein and stationary phase data alone and have to be established with experiments.
In order to get an advanced insight into events during a chromatographic experiment and for the development of suitable models for the description and optimization of these processes more detailed mass transport and adsorption measurements are needed. Classical methods utilize data gathered from the effluent of packed beds under linear or non-linear conditions. Contributions of single factors that play a role in mass transfer and adsorption cannot be analyzed independently from each other. The actual separation takes place inside the porous stationary phase, which cannot be directly analyzed with classical methods.
Confocal laser scanning microscopy is a new method for the analysis of mass transport and adsorption phenomena in chromatographic processes on the single particle level. The goal of this thesis is to establish the quantitative analysis of mass transfer and adsorption processes with confocal laser scanning microscopy. The method will be expanded to determine mass transport parameters, to analyze dynamic processes in packed beds and to measure mass transport phenomena during elution processes.
The thesis contains two parts: in the first part partitioning and mass transport phenoma under non-binding conditions will be analyzed. The second part deals with adsorption and mass transport processes under non-linear conditions and mass transport phenomena during a step elution.
DA  - 2006
KW  - Chromatographiesäule , Gelchromatographie , Chromatographie , Proteine , Proteintransport , Konfokale Mikroskopie , Simulation ,  , Protein , Mass transport , Confocal laser scanning microscopy , Chromatography , Simulation
LA  - ger
PY  - 2006
TI  - Untersuchungen zum Stofftransport von Proteinen in chromatographischen Medien mittels konfokaler Laser-Raster-Mikroskopie
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:361-10888
Y2  - 2024-12-24T18:47:51
ER  -