TY  - JOUR
AB  - Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65.
DA  - 2013
DO  - 10.1371/journal.pone.0065280
LA  - eng
IS  - 6
PY  - 2013
SN  - 1932-6203
T2  - PLoS ONE
TI  - Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-25772653
Y2  - 2024-11-21T21:57:49
ER  -