TY  - JOUR
AB  - L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum. In this study, C. glutamicum was engineered for overproduction of L-citrulline as major product without L-arginine being produced as by-product. To this end, L-arginine biosynthesis was derepressed by deletion of the arginine repressor gene argR and conversion of L-citrulline towards L-arginine was avoided by deletion of the argininosuccinate synthetase gene argG. Moreover, to facilitate L-citrulline production the gene encoding a feedback resistant N-acetyl L-glutamate kinase argBfbr as well as the gene encoding L-ornithine carbamoylphosphate transferase argF were overexpressed. The resulting strain accumulated 44.1 ± 0.5 mM L-citrulline from glucose minimal medium with a yield of 0.38 ± 0.01 g⋅g−1 and a volumetric productivity of 0.32 ± 0.01 g⋅l−1⋅h−1. In addition, production of L-citrulline from the alternative carbon sources starch, xylose, and glucosamine could be demonstrated.
DA  - 2014
DO  - 10.1186/s13568-014-0085-0
KW  - L-citrulline
KW  - Corynebacterium glutamicum
KW  - L-arginine
KW  - Alternative carbon sources
KW  - Starch
KW  - Metabolic engineering
KW  - Xylose
KW  - Glucosamine
LA  - eng
IS  - 1
PY  - 2014
SN  - 2191-0855
T2  - AMB Express
TI  - L-citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-27051036
Y2  - 2024-12-26T05:52:17
ER  -