TY  - JOUR
AB  - Background

The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the Pi starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ΔphoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR.
Results

DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to pstS promoter DNA in vitro. Gel mobility shift assays and mutational analysis of the pstS promoter region revealed that RamB binds to two sites localized at positions −74 to −88 and −9 to +2 with respect to the transcriptional start site of pstSCAB. Reporter gene studies supported the in vivo relevance of both binding sites for activation of pstSCAB by RamB. DNA microarray analysis revealed that expression of many Pi starvation genes reached higher levels during the Pi starvation response on minimal medium with glucose as sole carbon source than in Pi starved acetate-grown C. glutamicum cells.
Conclusions

In C. glutamicum, RamB is involved in expression control of pstSCAB operon. Thus, transcriptional regulation of pstSCAB is complex involving activation by the phosphate-responsive two-component regulatory system PhoSR and the regulators of carbon metabolism GlxR and RamB.
DA  - 2015
DO  - 10.1186/s12866-015-0437-1
KW  - Phosphate starvation
KW  - Corynebacterium glutamicum
KW  - pstS
KW  - RamB
KW  - Phosphorus metabolism
KW  - Carbon metabolism
KW  - Acetate metabolism
KW  - PhoR
KW  - GlxR
LA  - eng
IS  - 1
PY  - 2015
SN  - 1471-2180
T2  - BMC Microbiology
TI  - Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-27340724
Y2  - 2024-11-22T07:11:02
ER  -