TY  - JOUR
AB  - The development of tools has always been a major
driving force for the advancement of science. Optical microscopes
were the first instruments that allowed discovery and
descriptive studies of the subcellular features of microorganisms.
Although optical and electron microscopes remained at
the forefront of microbiological research tools since their inventions,
the advent of molecular genetics brought about
questions which had to be addressed with new Bgenetic tools^.
The unicellular green microalgal genus Chlamydomonas, especially
the most prominent species C. reinhardtii, has become
a frequently used model organism for many diverse
fields of research and molecular genetic analyses of
C. reinhardtii, as well as the available genetic tools and techniques,
have become increasingly sophisticated throughout
the last decades. The aim of this review is to provide an overview
of the molecular key features of C. reinhardtii and summarize
the progress related to the development of tools and
techniques for genetic engineering of this organism, from
pioneering DNA transformation experiments to state-of-theart
techniques for targeted nuclear genome editing and highthroughput
screening approaches.
DA  - 2015
DO  - 10.1007/s00253-015-6698-7
KW  - Chlamydomonas reinhardtiiGenetic engineeringGene silencingMolecular toolkitPromotersSelectable markers
LA  - eng
IS  - 13
M2  - 5407
PY  - 2015
SN  - 0175-7598
SP  - 5407-5418
T2  - Applied Microbiology and Biotechnology
TI  - Genetic tools and techniques for Chlamydomonas reinhardtii
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-27531659
Y2  - 2024-11-22T04:05:02
ER  -