TY  - JOUR
AB  - Background

The production of biogas takes place under anaerobic conditions and involves microbial decomposition of organic matter. Most of the participating microbes are still unknown and non-cultivable. Accordingly, shotgun metagenome sequencing currently is the method of choice to obtain insights into community composition and the genetic repertoire.
Findings

Here, we report on the deeply sequenced metagenome and metatranscriptome of a complex biogas-producing microbial community from an agricultural production-scale biogas plant. We assembled the metagenome and, as an example application, show that we reconstructed most genes involved in the methane metabolism, a key pathway involving methanogenesis performed by methanogenic Archaea. This result indicates that there is sufficient sequencing coverage for most downstream analyses.
Conclusions

Sequenced at least one order of magnitude deeper than previous studies, our metagenome data will enable new insights into community composition and the genetic potential of important community members. Moreover, mapping of transcripts to reconstructed genome sequences will enable the identification of active metabolic pathways in target organisms.
DA  - 2015
DO  - 10.1186/s13742-015-0073-6
KW  - Biogas
KW  - Metatranscriptomics
KW  - Sequencing
KW  - Metagenomics
KW  - Anaerobic digestion
KW  - Wet fermentation
KW  - Methanogenesis
KW  - Assembly
LA  - eng
IS  - 1
PY  - 2015
SN  - 2047-217x
T2  - GigaScience
TI  - Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-27649068
Y2  - 2024-11-21T23:17:02
ER  -