TY  - JOUR
AB  - Picosecond optical parametric oscillators (OPOs) with broad wavelength tunability are frequently used as light sources in hyperspectral coherent Raman scattering (CRS) microscopy. We investigate how changes in the pulse length during OPO wavelength tuning of the pump beam affect hyperspectral CRS imaging. We find that significant distortions of the resulting CRS spectra occur if the OPO is operated without monitoring pulse length variations. By utilizing a custom-written MATLAB based control program to counteract changes in pulse length, normalized and reproducible data sets can be acquired. We demonstrate this by comparing hyperspectral data obtained from pure substances, as well as relevant biological specimens.
DA  - 2018
DO  - 10.1364/oe.26.028312
LA  - eng
IS  - 22
M2  - 28312
PY  - 2018
SN  - 1094-4087
SP  - 28312-28322
T2  - Optics Express
TI  - Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29316098
Y2  - 2024-11-22T03:12:26
ER  -