TY  - JOUR
AB  - A novel technique to study protein synthesis is proposed that uses magnetic nanoparticles in combination with microfluidic devices to achieve new insights into translational regulation. Cellular protein synthesis is an energy-demanding process which is tightly controlled and is dependent on environmental and developmental requirements. Processivity and regulation of protein synthesis as part of the posttranslational nano-machinery has now moved back into the focus of cell biology, since it became apparent that multiple mechanisms are in place for fine-tuning of translation and conditional selection of transcripts. Recent methodological developments, such as ribosome foot printing, propel current research. Here we propose a strategy to open up a new field of labelling, separation, and analysis of specific polysomes using superparamagnetic particles following pharmacological arrest of translation during cell lysis and subsequent analysis. Translation occurs in polysomes, which are assemblies of specific transcripts, associated ribosomes, nascent polypeptides, and other factors. This supramolecular structure allows for unique approaches to selection of polysomes by targeting the specific transcript, ribosomes, or nascent polypeptides. Once labeled with functionalized superparamagnetic particles, such assemblies can be separated in microfluidic devices or magnetic ratchets and quantified. Insights into the dynamics of translation is obtained through quantifying large numbers of ribosomes along different locations of the polysome. Thus, an entire new concept for in vitro, ex vivo, and eventually single cell analysis will be realized and will allow for magnetic tracking of protein synthesis.
DA  - 2019
DO  - 10.3390/nano9040585
KW  - Arabidopsis thaliana
KW  - magnetic field
KW  - nanobead
KW  - polysome
KW  - protein synthesis
KW  - ribosome
KW  - superparamagnetic particle
KW  - translation
LA  - eng
IS  - 4
PY  - 2019
SN  - 1936-2625
T2  - Nanomaterials
TI  - Magnetic tracking of protein synthesis in microfluidic environments - challenges and perspectives
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29348725
Y2  - 2024-11-22T04:48:27
ER  -