TY  - JOUR
AB  - Nodulin 26-like-intrinsic-proteins (NIPs) play essential roles in transporting the nutrients, silicon and boron, in seed plants, but the evolutionary origin of this transport function and the co-permeability to toxic arsenic remains enigmatic. Horizontal gene transfer of a yet uncharacterized bacterial AqpN-aquaporin group was the starting-point for plant NIP evolution. We combined intense sequence-, phylogenetic and genetic context analyses and a mutational approach with various transport assays in oocytes and plants to resolve the trans-organismal and functional evolution of bacterial and algal and terrestrial plant NIPs and to reveal their molecular transport specificity features. We discovered that aqpN genes are prevalently located in arsenic-resistance-operons of various prokaryotic phyla. We provided genetic and functional evidence that these proteins contribute to the arsenic detoxification machinery. We identified NIPs with the ancestral bacterial AqpN selectivity filter composition in algae, liverworts, moss, hornworts and ferns and demonstrated that these archetype plant NIPs and their prokaryotic progenitors are almost impermeable to water and silicon but transport arsenic and boron. With a mutational approach, we demonstrated that during evolution, ancestral NIP selectivity shifted to allow subfunctionalizations. Together, our data provided evidence that evolution converted bacterial arsenic efflux channels into essential seed plant nutrient transporters. © 2019 The Authors New Phytologist © 2019 New Phytologist Trust.
DA  - 2020
DO  - 10.1111/nph.16217
LA  - eng
IS  - 3
M2  - 1383
PY  - 2020
SN  - 0028-646x
SP  - 1383-1396
T2  - New Phytologist
TI  - Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29377514
Y2  - 2024-11-23T19:00:35
ER  -