TY  - JOUR
AB  - Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work, we present a multiplane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multiplane image splitting prism combined with highspeed SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally highly resolved 3D image stack. We demonstrate the performance of multiplane SIM by applying this instrument to imaging the dynamics of mitochondria in living COS-7 cells.
DA  - 2020
DO  - 10.1515/nanoph-2019-0346
KW  - super-resolution optical microscopy
KW  - PACS10
KW  - 42.30.Wb
KW  - multiplane image
KW  - acquisition
KW  - structured illumination microscopy
LA  - eng
IS  - 1
M2  - 143
PY  - 2020
SN  - 2192-8606
SP  - 143-148
T2  - Nanophotonics
TI  - High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29407078
Y2  - 2024-11-22T00:47:18
ER  -