TY  - JOUR
AB  - Methanol is a sustainable substrate for biotechnology. In addition to natural methylotrophs, metabolic engineering has gained attention for transfer of methylotrophy. Here, we engineered Corynebacterium glutamicum for methanol-dependent growth with a sugar co-substrate. Heterologous expression of genes for methanol dehydrogenase from Bacillus methanolicus and of ribulose monophosphate pathway genes for hexulose phosphate synthase and isomerase from Bacillus subtilis enabled methanol-dependent growth of mutants carrying one of two independent metabolic cut-offs, i.e., either lacking ribose-5-phosphate isomerase or ribulose-5-phosphate epimerase. Whole genome sequencing of strains selected by adaptive laboratory evolution (ALE) for faster methanol-dependent growth was performed. Subsequently, three mutations were identified that caused improved methanol-dependent growth by (1) increased plasmid copy numbers, (2) enhanced riboflavin supply and (3) reduced formation of the methionine-analogue O-methyl-homoserine in the methanethiol pathway. Our findings serve as a foundation for the engineering of C. glutamicum to unleash the full potential of methanol as a carbon source in biotechnological processes.
DA  - 2020
DO  - 10.3390/ijms21103617
KW  - synthetic methylotrophy
KW  - methanol
KW  - ribulose monophosphate pathway
KW  - adaptive laboratory evolution
KW  - isotopic labeling
KW  - metabolic engineering
LA  - eng
IS  - 10
PY  - 2020
T2  - International Journal of Molecular Sciences
TI  - Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29434519
Y2  - 2024-11-22T04:06:08
ER  -